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    ROSIN LAB

    IMMUNE REGULATION OF
    DEVELOPMENTAL PROGRAMS



    DEPARTMENT OF ORAL BIOLOGICAL & MEDICAL SCIENCES

    FACULTY OF DENTISTRY

    THE UNIVERSITY OF BRITISH COLUMBIA

    OUR OBJECTIVE IS TO UNDERSTAND HOW DISTINCT POPULATIONS OF IMMUNE CELLS SIGNAL TO NEARBY CELLS DURING EMBRYOGENESIS TO CONTRIBUTE TO DEVELOPMENT

    We employ a variety of experimental approaches to identify unique cell types and the physical means through which they interact with or molecules they use to signal to nearby cells within their surroundings. By disrupting these intricate cellular interactions using genetic knockout lines, pharmacological inhibitors, or environmental insults that perturb the system, we are able study how altering normal developmental programs impacts circuitry and behaviour.

    REGULATORY PROGRAMS

    Understand the regulatory landscape for key genes and how they change across development

    Experimental Approach:

    ATAC-seq

    Bioinformatic Analysis

    Recombineering

    Pronuclear Injections

    Transgenic Reporter Assays

    Luciferase Assays

    MOLECULAR IDENTIFICATION

    Identification of unique cell types and discrete subpopulations of cells with distinct physiologies

    Experimental Approach:

    Single-Cell RNA-Sequencing

    Mass Spectrometry

    Metabolomics

    Proteomics

    In Situ Hybridization

    Immunohistochemistry

    Confocal Microscopy

    CELLULAR INTERACTIONS & SIGNALLING

    Identify key cellular interactions and signalling molecules that are used to communicate to nearby cells within unique micro-environments

    Experimental Approach:

    Ex Vivo Slice Culture

    ELISAs

    Sphere Assays

    Live-Cell Imaging

    Super-Resolution Microscopy

    Genetic Knockout Lines

    Pharmacological Inhibitors

    In Utero Electroporation

    ANALYSIS OF CIRCUITRY

    Understand the structural connectivity and physical wiring that is used by cells to communicate to one another by mapping new cell types, subpopulations, and signalling molecules onto circuits  

    Experimental Approach:

    Transgenic Reporters

    Viral Labeling

    CLARITY – Tissue Clearing

    Light-Sheet Microscopy

    BEHAVIOURAL ASSESSMENT

    Employ unique behavioural paradigms to assess various aspects of cognition, activity, memory, movement, and social interactions as a output to study cellular disruptions and defects in circuitry

    Experimental Approach:

    Open Field

    Elevated Plus Maze (EPM)

    Rotarod

    3 Chamber Sociability

    Marble Burying

    Novel Object

    Rosin Lab
    Vancouver Campus
    2350 Health Sciences Mall
    Life Sciences Centre (LSC) 3.320
    Vancouver, British Columbia Canada V6T 1Z3
    Website rosinlab.dentistry.ubc.ca
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